Second Harmonic Generation

Second harmonic generation is a nonlinear optical process where two photons interacting with certain materials combine into one photon at twice the frequency. The animation presents that optical behavior as a visual sequence of laser pulses, sample interaction, and signal collection.

Multiphoton Microscopy

Multiphoton microscopy uses simultaneous photon absorption to excite a sample with tightly localized energy. The animation emphasizes how that focused interaction allows imaging deeper into material while reducing out-of-focus excitation.

Confocal Microscopy

Confocal microscopy uses focused illumination and a pinhole to reject out-of-focus light. This animation shows the scanning and filtering logic that lets the system build clearer optical sections from a sample.